Instruments


AppliedPhotophysics CHIRASCAN PLUS CD SPECTROMETER
CD Cell light pathway as low as 0.05 mm
Simultaneoulsy measures CD spectra and absorbance 
Temperature ramping 4 °C - 100 °C up to 4 °C/min
Wavelength 170nm to 1150nm

Data analyzing software
Wyatt Technology DynaPro Plate Reader II
DLS With use of Dynamic Light Scattering technology proteins samples can be screened prior to crystallization trials.

Experiment can provide information on purity (dispersity) of the samples, 
protein complexes formation, and aggregation of proteins. 


Temperature range 4 °C - 55 °C with temperature ramping

TA Instruments Nano-ITC
NITC

24K gold reaction cell of 170 µl volume
Injection syringe volume 50 µl
Temperature range 2 °C - 80 °C
Multiple injection mode
Continuous injection mode

System includes sample and buffer degassing system

Data integration software on site

Please refer to BUFFER PREPARATION GUIDE before experiment set-up

BUFFER PREPARATION GUIDE

ART Robbins Instruments GRYPHON Robot
GRYPHON Fast set up of crystallization plates
 
Sitting drop size as low as 0.6 µl
Douglas Instruments Ltd ORYX Seeding Robot
ORYX Automated Robot for Crystal Drop Seeding
 
Intelliplate compatible
Bruker CRYSTAL FARM IMAGING SYSTEM
CRYSTAL FARM

Crystal Farm is set-up currently to 9°C

Automated imaging for crystallography condition hits

On-line access to view plate and well images

Available imaging with polarization filters

ONLINE ACCESS TO CRYSTAL FARM

(Requires VPN if access from outside UofS servers)

Beckman Coulter BIOMEK FX
BIOMEK Dual Arm Automated Robot for Liquid Handling
 
96-well and 8-span arms for precise liquid dispensing
 
Compatible with wide range of labware


Rigaku Alchemist DT Benchtop Liquid Handling System
ALCHEMIST Benchtop Liquid Handling System
 
Preparation of solutions for crystallography
 
10 ml tube format and plate format
 
Gradient Screen Preparation
 
No cross-contamination between solutions


Reichert Surface Plasmon Resonance with Automated Sample Delivery
ALCHEMIST Surface Plasmon Resonance instrument with automated sample delivery
 
Two channel system
 
Measuring the protein-protein and protein-small molecule binding constants
 
Several types of chips are available from the manufacturer at a relatively low cost
 


Wyatt Multi-Angle Light Scattering with Refractive index for MW determination in solution
ALCHEMIST Multi-Angle Light Scattering System with Refracting Index Measurement
 
Determination of molecular weight of protein in solution
 
Works independently or with the size exclusion column
 
Requires running ACTA FPLC with a programmed method
 


BRUKER Single Crystal Diffractometer with MOS area Detector
ALCHEMIST BRUKER single crystal diffractometer with the CMOS area detector
 
Evaluation of quality of crystal diffraction
 
Collecting X-ray diffraction data from protein and small molecule crystals
 
Crystal has to be mounted on a pin that fits the goniometer head
 
Temperturae of the crystal can be set between 100 K - 300 K


Photon Technologies International Quanta Master 40 Spectrofluorometer
QuarterMaster Photon Technologies International Quanta Master 40 Spectrofluorometer
 
Measure steady state rotational anisotropy (fluorescence depoloarization)
 
Measure steady state FRET (bulk assays)
 
Detects fluorophore emissions above 300 nm

For more information and to request access contact:
Dr. Linda Chelico,
tel. (306) 966-4318,
email: linda.chelico@usask.ca